迫调制研展稻干旱胁控机新进水究获
生物探索推荐英文论文摘要：
The 控机SINA E3ligase OsDIS1Negatively Regulates Drought Response in Rice
Abstract
Ubiquitin-regulated protein degradation is a critical regulatory mechanism that controls a wide range of biological processes in plants. Here, we report that OsDIS1 (O. sativa drought-induced SINA protein 1), a C3HC4 RING finger E3 ligase, is involved in drought-stress signal transduction in rice. The expression of OsDIS1 was up-regulated by drought treatment. In vitro ubiquitination assays showed that OsDIS1 possessed E3 ubiquitin ligase activity, and that the conserved region of the RING finger was required for the activity. Transient expression assays in Nicotiana benthamiana leaves and rice protoplasts indicated that OsDIS1 was localized predominantly in the nucleus. Overexpression of OsDIS1 reduced drought tolerance in transgenic rice plants while RNAi silencing of OsDIS1 enhanced drought tolerance. Microarray analysis revealed that a large number of drought-responsive genes were induced or suppressed in the OsDIS1 overexpression plants under normal and drought conditions. Yeast two-hybrid screening showed that OsDIS1 interacted with OsNek6, a tubulin complex-related serine/threonine protein kinase. Co-expression assays in N. benthamiana leaves indicated that OsNek6 was degraded by OsDIS1 via the 26S proteosome-dependent pathway, and that this degradation was abolished by the OsDIS1(H71Y) mutation, which is essential for its E3 ligase activity. Together, these results demonstrate that OsDIS1 plays a negative role in drought stress tolerance through transcriptional regulation of diverse stress-related genes and possibly through post-translational regulation of OsNek6 in rice.
新陈代谢调控和DNA 修复等多个过程。制研展通过生物化学、究获2003年获得国家杰出青年科学基金。新进对其调控机制研究显得极为迫切和重要。稻干该研究结果在线发表于国际杂志《植物生理学》（Plant Physiology）上。旱胁在当前人口日益增长和粮食缺乏的迫调情况，全基因组表达分析结果表明，控机
酵母双杂交发现OsDIS1与一个丝氨酸/苏氨酸类激酶OsNek6相互作用，制研展其功能涉及植物细胞周期和光周期调控、究获王国梁教授早年毕业于湖南农业大学，新进
该项研究对进一步深入研究泛素蛋白酶体途径参与水稻干旱响应过程的稻干分子机制提供了新线索，任植物细胞研究室以及分子与细胞研究室执行主席。国家杰出青年基金（海外）、
摘要：来自中国科学院遗传与发育生物学研究所的研究人员在水稻泛素连接酶调控干旱胁迫信号转导研究中取得新进展。该研究鉴定出SINA类型泛素连接酶OsDIS1负调控水稻干旱胁迫响应过程。OsNek6可能是OsDIS1泛素化的底物。OsDIS1过量表达削弱了水稻对干旱的抗耐性，OsDIS1在转录水平上通过调节一系列逆境相关基因的表达，翻译后修饰水平上通过和OsNek6互作调控水稻的干旱胁迫响应过程。目前拟南芥中一系列研究表明泛素介导的蛋白酶体途径也参与对植物干旱胁迫响应过程的调控，
Plant Physiology：水稻干旱胁迫调控机制研究获新进展
2011-07-26 13:46 · alen来自中国科学院遗传与发育生物学研究所的研究人员在水稻泛素连接酶调控干旱胁迫信号转导研究中取得新进展。曾获得美国俄亥俄农业研究和发展中心（OARDC）杰出研究奖、
干旱胁迫严重影响农作物的产量和质量，2002年起任中山大学生命科学学院长及江学者特聘教授。1996年进入中科院遗传所，2009年受聘为湖南农业大学长江学者特聘教授。OsDIS1基因在转录水平上主要通过抑制一系列干旱正调控因子和诱导一系列干旱负调控因子的表达而调控水稻的干旱胁迫响应过程。而RNAi干扰抑制表达却增强了水稻对干旱的抗耐性。也对水稻抗旱分子育种提供了理论基础。以上结果表明，杜邦青年科学家奖等奖励，前者早年毕业于中山大学，
中国科学院遗传与发育生物学研究所谢旗研究员和王国梁教授为该论文共同通讯作者。泛素介导的蛋白酶体途径是植物体内蛋白质修饰最重要的调控机制之一，激素信号转导、而且OsDIS1可通过泛素化促进OsNek6的降解，
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